Optimized multi-view imaging improves the observation of optically manipulatednon-spherical particles
Identifieur interne : 000630 ( Main/Exploration ); précédent : 000629; suivant : 000631Optimized multi-view imaging improves the observation of optically manipulatednon-spherical particles
Auteurs : L. Selvaggi [Italie] ; E. Ferrari [Italie] ; A. Moradi [Iran] ; S C Santucci [Italie] ; P. Beuzer [Italie, France] ; D. Cojoc [Italie]Source :
- Journal of Optics [ 2040-8978 ] ; 2010.
Abstract
We report the optimization of two-side sample imaging through twoorthogonal objectives and sample manipulation by single-beam opticaltrapping through one of the objectives. A high numerical aperture objective(NA 1.0) is used to generate multiple laser traps configured by phase diffractive optical elementsimplemented on a phase programmable modulator. Supplementary lateral imaging through aNA 0.55 objective represents a trade-off between the resolution and the field of view that providesadditional information about the sample and its environment, impossible to obtain fromconventional optical trapping setups where imaging is performed only along the trappingobjective axis. The usefulness of two-side imaging combined with optical manipulation isillustrated by three different experiments: multiple beads trapped in 3D arrays, red bloodcells rotated by multiple tweezers and manipulation of a glass grain with irregular shape.
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DOI: 10.1088/2040-8978/12/3/035303
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Selvaggi</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Optical Manipulation Lab, CNR-INFM Laboratorio Nazionale TASC, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Universit della Calabria, 87036 Arcavacata di Rende (CS)</wicri:regionArea><wicri:noRegion>87036 Arcavacata di Rende (CS)</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Italie</country></affiliation></author><author><name sortKey="Ferrari, E" sort="Ferrari, E" uniqKey="Ferrari E" first="E" last="Ferrari">E. 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Moradi</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Institute for Advanced Studies in Basic Sciences, Gava Zang, Zanjan 45195-1159</wicri:regionArea><wicri:noRegion>Zanjan 45195-1159</wicri:noRegion></affiliation><affiliation><wicri:noCountry code="no comma">These authors contributed equally to this work</wicri:noCountry></affiliation></author><author><name sortKey="Santucci, S C" sort="Santucci, S C" uniqKey="Santucci S" first="S C" last="Santucci">S C Santucci</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>Optical Manipulation Lab, CNR-INFM Laboratorio Nazionale TASC, Trieste</wicri:regionArea><wicri:noRegion>Trieste</wicri:noRegion></affiliation></author><author><name sortKey="Beuzer, P" sort="Beuzer, P" uniqKey="Beuzer P" first="P" last="Beuzer">P. 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Opt.</title><idno type="ISSN">2040-8978</idno><imprint><publisher>IOP Publishing</publisher><date type="published" when="2010">2010</date><biblScope unit="volume">12</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="6">6</biblScope><biblScope unit="production">Printed in the UK</biblScope></imprint><idno type="ISSN">2040-8978</idno></series><idno type="istex">28975F3FC2B97CF37B2C8D8589922131D869440E</idno><idno type="DOI">10.1088/2040-8978/12/3/035303</idno><idno type="PII">S2040-8978(10)38355-8</idno><idno type="articleID">338355</idno><idno type="articleNumber">035303</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">2040-8978</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">We report the optimization of two-side sample imaging through twoorthogonal objectives and sample manipulation by single-beam opticaltrapping through one of the objectives. A high numerical aperture objective(NA 1.0) is used to generate multiple laser traps configured by phase diffractive optical elementsimplemented on a phase programmable modulator. Supplementary lateral imaging through aNA 0.55 objective represents a trade-off between the resolution and the field of view that providesadditional information about the sample and its environment, impossible to obtain fromconventional optical trapping setups where imaging is performed only along the trappingobjective axis. The usefulness of two-side imaging combined with optical manipulation isillustrated by three different experiments: multiple beads trapped in 3D arrays, red bloodcells rotated by multiple tweezers and manipulation of a glass grain with irregular shape.</div></front></TEI><affiliations><list><country><li>France</li><li>Iran</li><li>Italie</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="Italie"><noRegion><name sortKey="Selvaggi, L" sort="Selvaggi, L" uniqKey="Selvaggi L" first="L" last="Selvaggi">L. Selvaggi</name></noRegion><name sortKey="Beuzer, P" sort="Beuzer, P" uniqKey="Beuzer P" first="P" last="Beuzer">P. Beuzer</name><name sortKey="Cojoc, D" sort="Cojoc, D" uniqKey="Cojoc D" first="D" last="Cojoc">D. Cojoc</name><name sortKey="Ferrari, E" sort="Ferrari, E" uniqKey="Ferrari E" first="E" last="Ferrari">E. Ferrari</name><name sortKey="Santucci, S C" sort="Santucci, S C" uniqKey="Santucci S" first="S C" last="Santucci">S C Santucci</name><name sortKey="Selvaggi, L" sort="Selvaggi, L" uniqKey="Selvaggi L" first="L" last="Selvaggi">L. Selvaggi</name><name sortKey="Selvaggi, L" sort="Selvaggi, L" uniqKey="Selvaggi L" first="L" last="Selvaggi">L. Selvaggi</name></country><country name="Iran"><noRegion><name sortKey="Moradi, A" sort="Moradi, A" uniqKey="Moradi A" first="A" last="Moradi">A. Moradi</name></noRegion></country><country name="France"><region name="Île-de-France"><name sortKey="Beuzer, P" sort="Beuzer, P" uniqKey="Beuzer P" first="P" last="Beuzer">P. Beuzer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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